Oligo purification equipmnt rau Oligo primer synthesis
Oligo purification equipmnt rau Oligo primer synthesis,
oligo synthesis txhawb tshuab,
Specification
| Compatible boards | 1, 3, 5, 8. |
| Lim | tshuab filtration, suction pom |
| Tus naj npawb ntawm cov chaw txhaj tshuaj | 5, 6, 7, 8, 9, 10. |
| Tau tshaj phaj hom | C18 phaj, sib sib zog nqus phaj phaj, hluavtaws phaj (tshaj nrog feem ntau cov phaj hluavtaws), microtiter phaj |
| Module | ib axis los yog dual axis |
| Qhov hluav taws xob | 220 V |
| Warranty | 1 xyoo |
| Kev cai | Txais |
Txoj kev sib txawv ntawm Purification
1. Gel Electrophoresis Chromatography Purification
Siv denaturing polyacrylamide gel electrophoresis chromatography rau purification.Tus neeg sawv cev denaturing feem ntau yog 4M formamide lossis 7M urea, qhov concentration ntawm acrylamide yog nyob nruab nrab ntawm 5-15%, thiab feem ntau ntawm methacrylamide yog feem ntau ntawm 2-10%.
Tom qab electrophoresis, txoj hauj lwm ntawm nucleic acid band yuav tsum tau txiav txim siab nyob rau hauv lub irradiation ntawm ultraviolet lub teeb, lub gel uas muaj lub hom phiaj nucleic acid raug txiav tawm, lub nucleic acid yog smashed thiab leached, thiab ces cov leaching tov yog concentrated, desalted, quantified thiab lyophilized.
2. DMT-On, HPLC Purification
Xaiv hom DMT-On thaum lub sij hawm kev sib txuas, cov khoom siv crude yog centrifuged thiab concentrated nyob rau hauv chav tsev kub kom tshem tawm ntau tshaj ammonia tom qab cov aminolysis.
Kev sib cais tau ua tiav siv C18 kem nrog acetonitrile thiab 10% triethylamine-acetic acid (TEAA) raws li eluent.Tom qab qhov elution tiav lawm, nws yog concentrated, thiab tom qab ntawd pawg DMT raug tshem tawm nrog trifluoroacetic acid.Tom qab neutralization, qee cov ntsev thiab me me molecules raug tshem tawm los ntawm lub raj txiav, thiab thaum kawg desalted.
Txoj kev no tuaj yeem tau txais cov khoom lag luam nrog ntau dua purity, tab sis nws yuav tsum tau them sai sai rau qhov tshwm sim ntawm depurination.
3. DMT-Off, HPLC Purification
Xaiv lub DMT-Off thaum lub sij hawm synthesis, thiab cov khoom crude yog centrifuged thiab concentrated nyob rau hauv chav tsev kub kom tshem tawm tshaj ammonia tom qab ammonolysis.
Kev sib cais tau ua tiav siv C18 kem nrog acetonitrile thiab 10% triethylamine-acetic acid hauv dej raws li eluent.Tom qab kev sib cais tiav thiab ntsuas, cov aliquots yog lyophilized.
Txoj kev no yuav tsum tau ua tib zoo hloov kho ntawm kev sib cais, thiab tuaj yeem tau txais cov hom phiaj tseem ceeb.
oligo synthesis accerry khoom rau dna rna synthesis voj voog, uas haum rau ib txwm primer, thiab lwm yam luv luv saw primer, ua rau oligo siab purity.
Tuam Tshoj chaw tsim tshuaj paus ntawm oligo synthesis khoom nrog zoo zoo thiab kev pab zoo tshaj plaws.
