Ob leeg DNA thiab RNA synthesis yog raws li lub zog-theem synthesis lub tswv yim thiab phosphoramidite chemistry, DNA synthesizer tuaj yeem siv los ua ke RNA lossis RNA analogues yam tsis muaj kev hloov pauv ntxiv, thiab cov reagents hauv DNA synthesis tuaj yeem siv ncaj qha rau hauv RNA thiab cov nucleic acids. ' synthesis.Raws li pom hauv daim duab 1, 2'-hydroxy hauv RNA phosphoramidite yog tiv thaiv nrog silyl tiv thaiv pab pawg, piv txwv li t-butyldimethylsilyl (TBDMS), tiv thaiv kev tawm tsam ntawm pawg 2'-hydroxy.Cov pab pawg TBDMS loj heev cuam tshuam cov tshuaj tiv thaiv ntawm phosphoramidite thiab 5'-hydroxy pawg ntawm cov khoom txhawb nqa, thiab lub sijhawm sib txuas ntev dua yuav tsum tau ua kom paub txog qhov ua tau zoo ntawm kev sib txuas.
Zoo ib yam li DNA synthesis, cov synthesized RNA yog thawj zaug cleavaged los ntawm cov khoom txhawb nqa los ntawm aminolysis, ces pawg TBDMS tau cleavaged los ntawm tetrabutylammonium fluoride (TBAF) los yog trimethylamine trihydrofluoride.Crude RNA tuaj yeem ua kom huv los ntawm recrystallized los ntawm cawv thiab HPLC.
Daim duab 1. Cov qauv tshuaj ntawm cov tsev hauv paus hauv DNA thiab RNA synthesis.
a) dABz phosphoramidite thiab b) rABz 2'-OTBDMS phosphoramidite.
Kev tsim cov tshuaj siRNA yuav tsum muaj cov qauv analogues ntawm haiv neeg RNA kom nce biocompatibility, 2'-hydroxy ntawm RNA tuaj yeem hloov los ntawm MeO, F thiab MOE pawg, thiab cov xauv nucleic acid (LNA) kuj muab kev ua haujlwm zoo hauv RNA kho. (Daim duab 2).Cov phosphoramidites muab cov haujlwm zoo sib xws li DNA hom phosphoramidites hauv kev sib txuas, thiab cov txheej txheem ua haujlwm thiab ua kom huv ntawm cov non-natural nucleic acids zoo ib yam li cov neeg DNA.
Daim duab 2. Cov qauv tshuaj ntawm cov tsev blocks hauv cov tshuaj siRNA.a) dABz 2-MeO phosphoramide;b) dABz 2-F phosphoramide;c) dABz 2-MOE phosphoramidite thiab d) dABz Xauv phosphoramidite.
Phosphoramidesyog qhov tseem ceeb rau kev sib txuas ntawm cov noob, suav nrog feem ntau ntawm DNA thiab RNA cov tsev neeg thiab lawv cov derivatives, tag nrho cov phosphoramidite saum toj no peb tuaj yeem muab rau hauv ib pob.
Peb niaj hnub txhim kho kev ua tau zoo ntawm peb cov khoom, ua tiav peb cov txheej txheem tsim khoom thiab tau txais cov ntsiab lus zoo.Peb tsis tsuas yog muab koj cov khoom zoo, tab sis kuj muab kev cob qhia thiab kev pabcuam rau koj.Peb tau nruab nrog cov kws tshaj lij kev tswj hwm cov neeg ua haujlwm thiab pab pawg ua haujlwm zoo thiab cov neeg ua haujlwm saib xyuas los muab peb cov neeg siv khoom nrog kev pabcuam zoo tom qab muag.
Post lub sij hawm: Aug-09-2022